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31.
32.
In vivo Kinetic Biodistribution of Nano‐Sized Outer Membrane Vesicles Derived from Bacteria 下载免费PDF全文
Su Chul Jang Sae Rom Kim Yae Jin Yoon Kyong‐Su Park Ji Hyun Kim Jaewook Lee Oh Youn Kim Eun‐Jeong Choi Dae‐Kyum Kim Dong‐Sic Choi Yoon‐Keun Kim Jaesung Park Dolores Di Vizio Yong Song Gho 《Small (Weinheim an der Bergstrasse, Germany)》2015,11(4):456-461
Evaluation of kinetic distribution and behaviors of nanoparticles in vivo provides crucial clues into their roles in living organisms. Extracellular vesicles are evolutionary conserved nanoparticles, known to play important biological functions in intercellular, inter‐species, and inter‐kingdom communication. In this study, the first kinetic analysis of the biodistribution of outer membrane vesicles (OMVs)—bacterial extracellular vesicles—with immune‐modulatory functions is performed. OMVs, injected intraperitoneally, spread to the whole mouse body and accumulate in the liver, lung, spleen, and kidney within 3 h of administration. As an early systemic inflammation response, increased levels of TNF‐α and IL‐6 are observed in serum and bronchoalveolar lavage fluid. In addition, the number of leukocytes and platelets in the blood is decreased. OMVs and cytokine concentrations, as well as body temperature are gradually decreased 6 h after OMV injection, in concomitance with the formation of eye exudates, and of an increase in ICAM‐1 levels in the lung. Following OMV elimination, most of the inflammatory signs are reverted, 12 h post‐injection. However, leukocytes in bronchoalveolar lavage fluid are increased as a late reaction. Taken together, these results suggest that OMVs are effective mediators of long distance communication in vivo. 相似文献
33.
Reducing the complexity of distance measurement methods for circular turbo codes that use structured interleavers 下载免费PDF全文
Youssouf Ould‐Cheikh‐Mouhamedou 《International Journal of Communication Systems》2015,28(9):1572-1579
The knowledge of turbo code's minimum Hamming distance (dmin) and its corresponding codeword multiplicity (Amin) is of a great importance because the error correction capability of a code is strongly tied to the values of dmin and Amin. Unfortunately, the computational complexity associated with the search for dmin and Amin can be very high, especially for a turbo code that has high dmin value. This paper introduces some useful properties of turbo codes that use structured interleavers together with circular encoding. These properties allow for a significant reduction of search space and thus reduce significantly the computational complexity associated with the determination of dmin and Amin values. © 2014 The Authors. International Journal of Communication Systems published by John Wiley & Sons, Ltd. 相似文献
34.
This article presents a new method for pairing devices securely. The commitment-based authentication uses a fuzzy secret that the devices only know approximately. Its novel feature is time-based opening of commitments in a single round. We also introduce a new source for the fuzzy secret: synchronized drawing with two fingers of the same hand on two touch screens or surfaces. The drawings are encoded as strings and compared with an edit-distance metric. A prototype implementation of this surprisingly simple and natural pairing mechanism shows that it accurately differentiates between true positives and man-in-the-middle attackers. 相似文献
35.
端点检测技术是语音信号处理的关键技术之一,为提高低信噪比环境下端点检测的准确率和稳健性,提出了一种非平稳噪声抑制和调制域谱减结合功率归一化倒谱距离的端点检测算法。该算法首先通过抑制非平稳噪声再采用调制域谱减消除残余噪声来提升信噪比,减少语音失真。然后再提取每帧信号的功率归一化倒谱系数,计算每帧信号与背景噪声的功率归一化倒谱距离。最后将该倒谱距离作为检测参数,采用双门限判决方法进行端点检测。实验结果表明,该端点检测算法对语音帧和噪声帧具有较好的区分性。此外,在低信噪比环境下,所提出的算法对于不同类型的噪声都具有较好的稳健性。 相似文献
36.
Since the discovery of biological antifreeze glycoproteins (AFGPs), which can inhibit ice nucleation, there has been considerable interest in understanding their mechanisms and mimicking them in synthetic polymers. In this study, we used molecular dynamics simulations of modified polyvinyl alcohol (PVA) compounds to show that the hydroxyl (OH) group distance is a key factor in whether certain compounds promote or inhibit ice nucleation. A hydroxyl distance smaller than ~2.8 Å but greater than ~7.1 Å in modified PVA (MPVA) compounds was associated with the promotion of ice nucleation, while a hydroxyl group separation distance of approximately ~5.0 Å was correlated with a delay in ice nucleation, owing to changes in the energy of the system. Thus, these results may help explain some of the mechanisms of current known anti-freeze compounds and may have implications for designing new anti-freeze compounds in the future. 相似文献
37.
38.
为了得到斜沟煤矿18205工作面CO2致裂爆破钻孔最优布置参数,提高煤层瓦斯透气性系数,依据物理爆破原理,推导出了二氧化碳致裂器爆破时产生裂隙圈半径的计算公式;并通过FLAC^3D数值模拟软件进行模拟,得出:爆破孔间距为7 m、爆破器间距为5 m时,液态CO2致裂爆破可实现最佳的增透效果,当爆破孔间距越大时,透气性系数提高幅度越大,煤层增透效果越显著,抽采效率越高。 相似文献
39.
Yuanming Wu Xiaohua Chen Li Wang Lunjie Wu Lu Lin Wenwu Ding Zhenming Che Jianfeng Wang Jianzhou Li Yi Liu Weifeng Sun 《International Journal of Food Science & Technology》2020,55(4):1825-1832
Preparation of flavour peptides with microorganisms is an attractive choice for its large-scale production. However, beefy meaty peptide (BMP), as a research hotspot in the field of umami peptide, has been studied in few microorganisms, and furthermore, the safe preparation of BMP has not yet been reported. In this study, multi-copy BMP (8BMP) was successfully expressed and produced in the ‘generally recognized as safe’ Bacillus subtilis (B. subtilis). Firstly, 8BMP with the potentially intense umami was screened out based on molecular docking analysis. Then, it was successfully expressed in the B. subtilis 168 and verified through sensory evaluation. Finally, the scaling capacity of 8BMP was evaluated in a 5-L fermenter, with the highest yield (0.84 g L−1) 6.4 times than that of shake flask, which is conductive for industrial production of BMP and other food peptides. 相似文献
40.
声波测试是当前测试煤矿深井巷道围岩松动圈范围的一种比较准确的方式。随着深井巷道的推进,巷道附近一定范围内的围岩由于前期爆破和后期围岩内部应力调整,会产生较多的裂隙和形变。而围岩的岩性、裂隙发育程度、应力分布情况都会显著影响声波在围岩中的传播速度。通过监测迎头推进过程中的围岩波速变化情况,分析了迎头推进距离、岩性、巷道位置等对围岩松动圈的影响。 相似文献